Human receptor for calicivirus binding detected using Retrogenix technology
A new paper, published in the journal mBio, describes the identification and validation of the specific human cell surface receptor for the Hom-1 virus, a member of the caliciviridae family of viruses that infect a broad range of animal hosts and are associated with various chronic and acute illnesses.
The study was led by scientists at the National Insititutes for Health (NIH) and Oregon State University in collaboration with Retrogenix.
The Retrogenix technology was used to screen Hom-1 virus-like particles (VLP) against extensive arrays of human plasma membrane proteins over-expressed in human cells. A single, highly specific interaction was identified between Hom-1 VLPs and the cell surface protein, human junctional adhesion molecule-1 (hJAM1). This interaction was confirmed through competitive binding assays as well as by inducing susceptibility to infection in nonpermissive cells. These results provide key information on the mechanism of human infection and further our understanding of species specificity within this family of viruses.
To find out more about the versatility of the Retrogenix technology in identifying primary receptors and off-targets for a variety of ligands please see the Applications pages.