Publication: The Use of Cell Microarray Technology for Rapid Identification of Therapeutic SARS-CoV-2 Protein Targets
A high throughput screening library approach was used to detect binding interactions of SARS-CoV-2 proteins, confirming a number of previously known receptors as well as the discovery of three novel receptors: NID1, CNTN1, and APOA4. With this knowledge, we may further understand disease pathogenesis of COVID-19 patients and how infection by SARS-CoV-2 may lead to failure of specific organs or virulence in different ethnicities. Importantly, we illustrate a methodology that can be used for rapid and unbiased identification of cell surface receptors to support drug screening and drug repurposing approaches for this and future pandemics.
Full-length SARS-CoV-2 spike trimer proteins were screened for binding to 5845 human HEK293 cell-expressed monomeric or heterodimeric human plasma membrane proteins and cell-surface tethered human secreted proteins using the Retrogenix cell microarray technology. This physiologically relevant system allows even low affinity interactions with the viral spike protein to be detected with a high degree of sensitivity and specificity.
Overall, our data suggests COVID-19 may be a highly promiscuous virus exploiting multiple mechanisms of cell entry, contributing to its severity and targeting of specific patient groups. Furthermore, using recombinant full-length spike trimer proteins coupled with cell microarray technology and flow cytometry, we have demonstrated a fast, accurate, and comprehensive approach for discovering the human cell surface targets of viruses.
Learn more about the use of this unique Cell Microarray Technology for comprehensive receptor profiling.