Physiological system for detecting ligand-receptor interactions

Identifying precisely how a biological or chemical entity interacts with the human cell is fundamental to furthering our understanding of both normal biological and disease processes as well as revealing the mechanism of action of drugs and their potential for toxicity. Retrogenix’s unique cell microarray technology provides a fast, accurate and effective solution for discovering the human cell surface and secreted protein targets of antibodies, proteins, viruses and small molecules. This allows our global pharmaceutical clients to:

• overcome the critical deconvolution step in phenotypic drug discovery
• uncover novel, high quality and exploitable drug targets
• explore safety liabilities of lead candidates using the most comprehensive human expressed off-target system

Retrogenix’s biologically-relevant system detects specific interactions with a high degree of sensitivity. This unique tool uses proprietary arrays of expression vectors – encoding over 6,200 full-length human plasma membrane and tethered secreted proteins – spotted onto slides. Human cells grown over the top become reverse-transfected resulting in cell surface expression of each respective protein at distinct slide locations. The test molecule is applied and specific binding analysed and confirmed using an appropriate detection system.